Mononucleosis Spot Test

Alternate Names

  • Mononuclear Heterphil Test
  • Heterophil Antibody Test
  • Monospot Test

Infectious mononucleosis is caused by the Epstein-Barr virus. Following exposure to the virus, the body will develop antibodies specific to the organism as it fights the infection. This blood test measures the amount of antibodies against Epstein-Barr virus that causes infectious mononucleosis, which usually affects young adults. The test most frequently used is the Monospot Test, since it is the quickest and easiest to run. Mononuclear heterophil test, or heterophil antibody test are other tests that may also be done.

There can be both false negative and positive results with this test, and the test should be repeated in people who have the symptoms of infectious mononucleosis, such as fever, sore throat, enlarged spleen, and enlarged lymph nodes. Other diseases associated with mononucleosis are chronic EBV, chronic liver disease, Burkitt's lymphoma, stomach or intestinal cancer, chronic fatigue syndrome, SLE, and leukemia.

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